Simultaneous Determination of Urinary Vanillylmandelic Acid and 5-Hydroxyindoleacetic Acid

Uniform chromatographic conditions for quantifying urinary catecholamines, metanephrines, vanillylmandelic acid, 5-hydroxyindoleacetic acid, by liquid chromatography, with electrochemical detection.

Uniform liquid-chromatographic conditions were developed such that we could quantify norepinephrine, epinephrine, normetanephrine, metanephrine, vanillylmandelic acid, and 5-hydroxyindoleacetic acid in urine by using a single mobile phase of monochloroacetic acid and citric acid, 0.1 mol/L each. All compounds were separated on a C18 column and detected electrochemically at a potential of +0.800...

Acetic acid interference with determination of 5-hydroxyindoleacetic acid obviated.

1. Pratt, J. J., Steroid immunoassay in clinical chemistry. Clin. Chem. 24, 1869 (1978). 2. Shackleton, C. H. L., and Whitney, J. 0., Use of Sep-pak cartridges for urinary steroid extraction: Evaluation of the method for use prior to gas chromatographic analysis. Clin. Chim. Acta (in press). 3. Thenot, J.-P., and Horning, E. C., MOTMS derivatives of human steroids for GC and GC-MS studies. Anal...

A Method for Quantitative Extraction of Urinary 5-hydroxyindoleacetic Acid.

HE QUANTITATIVE ESTIMATION of urinary 5-hydroxyindoleacetic acid (5-HIAA) has attained clinical importance with increasing interest in indole metabolism. Urinary 5-HIAA levels have largely been determined by the use of an extraction procedure described by Udenfriend et al. (1). The author has found, as have others (2), that this method produces inconsistent results on varying dilutions of a giv...

Urinary 5-hydroxyindoleacetic acid by HPLC with electrochemical detection.

Specific photometric determination of 5-hydroxyindoleacetic acid in urine.

Udenfriend ‘s nitrosonaphthol test for 5-hydroxyindoleacetic acid (5-HIAA) is subject to interference by urinary phenolic acids and drug metabolites. Studies in our laboratory have shown that the specificity and sensitivity of the nitrosonaphthol reaction with 5-hydroxyindoles can be greatly enhanced by the addition of 2-mercaptoethanol. This observation serves as the basis of a simple, improve...